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- Title
Carbazole/dioxin-degrading car gene cluster is located on the chromosome of Pseudomonas stutzeri strain OM1 in a form different from the simple transposition of Tn4676.
- Authors
Shintani, Masaki; Nojiri, Hideaki; Yoshida, Takako; Habe, Hiroshi; Omori, Toshio
- Abstract
The carbazole-degrading (car) operon on the chromosome of Pseudomonas stutzeri strain OM1 showed >99% identity to that in the 72.8 kb catabolic transposon, Tn4676, on plasmid pCAR1. Southern hybridization using probes prepared from the pCAR1 sequence and sequencing analyses showed that the OM1 chromosome contained the 55 kb DNA region, almost all of which was a part of Tn4676, flanked by two copies of novel insertion sequence, ISPst3, and included the car gene.
- Subjects
PSEUDOMONAS; GENES; CARBAZOLE; OPERONS; CHROMOSOMES; METABOLISM; TRANSPOSONS
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 15, p1255
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1025079027730