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- Title
Dimethylsulfoxide and sodium butyrate enhance the production of recombinant cyclooxygenase 2 in stably transformed Drosophila melanogaster S2 cells.
- Authors
Hwa Chang, Kyung; Hwa Park, Jong; Hyung Lee, Youn; Ho Kim, Jong; Ok Chun, Hyung; Hak Kim, Je; Sik Chung, In
- Abstract
Recombinant human cyclooxygenase 2 (Cox 2) was expressed in stably transformed Drosophila melanogaster S2 cells, and was present primarily in the cellular fraction at a molecular weight of 70 to 74 kDa. Recombinant Cox 2 was purified using Ni2+-affinity fractionation to a specific activity of 24 800 U mg-1 protein. The peak level of recombinant Cox 2 production was 1.6 μg (107 cells)-1, seven days after induction with 0.5 mM CuSO4. Supplementing the cultures with dimethylsulfoxide or sodium butyrate increased recombinant Cox 2 production by 170% and 86%, respectively.
- Subjects
CYCLOOXYGENASE 2; RECOMBINANT proteins; ENZYME kinetics; DROSOPHILA melanogaster; FRUIT flies; CRYOSCOPY; ENZYMOLOGY
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 16, p1353
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1019841829667