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- Title
Fusaric acid induces a notochord malformation in zebrafish via copper chelation.
- Authors
Yin, Emily; Rakhmankulova, Malika; Kucera, Kaury; Sena Filho, Jose; Portero, Carolina; Narváez-Trujillo, Alexandra; Holley, Scott; Strobel, Scott
- Abstract
Over a thousand extracts were tested for phenotypic effects in developing zebrafish embryos to identify bioactive molecules produced by endophytic fungi. One extract isolated from Fusarium sp., a widely distributed fungal genus found in soil and often associated with plants, induced an undulated notochord in developing zebrafish embryos. The active compound was isolated and identified as fusaric acid. Previous literature has shown this phenotype to be associated with copper chelation from the active site of lysyl oxidase, but the ability of fusaric acid to bind copper ions has not been well described. Isothermal titration calorimetry revealed that fusaric acid is a modest copper chelator with a binding constant of 4.4 × 10 M. These results shed light on the toxicity of fusaric acid and the potential teratogenic effects of consuming plants infected with Fusarium sp.
- Subjects
COPPER chelates synthesis; ZEBRA danio embryos; NATURAL products; MYCOTOXINS; PLANT extracts; ENDOPHYTIC fungi; BIOACTIVE compounds
- Publication
BioMetals, 2015, Vol 28, Issue 4, p783
- ISSN
0966-0844
- Publication type
Article
- DOI
10.1007/s10534-015-9855-7