We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Protective effects of Radix Codonopsis on ischemia-reperfusion injury in rats after kidney transplantation.
- Authors
He, Bo; Zhang, Ying-Tian; Yuan, Xin-Gang; Sun, Jing-Song; Wei, Guang-Hui; Lin, Tao
- Abstract
<bold>Purpose: </bold>Kidney ischemia-reperfusion injury (IRI) after kidney transplant remains a major problem, separate from immune rejection that can lead to kidney transplant failure and graft function loss. Free radicals, disturbance of microcirculation and the inflammatory cascade appear to be the main contributors. Radix Codonopsis, a traditional Chinese drug used in vascular diseases, is an antioxidant and free radical scavenger. This study investigates the protective effect and underlying mechanisms of Radix Codonopsis extract saponins on kidney transplantation.<bold>Methods: </bold>Renal transplantation was performed after rat kidneys had been stored for 1 h at 4°C. Blood urea nitrogen (BUN), serum creatinine (Scr), superoxide dismutase (SOD) and malondialdehyde (MDA) were assayed; bcl-2 and bax mRNA expression was detected using RT-PCR; bcl-2 and bax protein expression was detected by immunohistochemistry (IHC). Terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) was used to detect apoptotic cells and determine the apoptosis index (AI). Analysis of variance (ANOVA) followed by Dunnett's test was used when more than two groups were compared.<bold>Results: </bold>Saponin-treated animals showed increased SOD levels accompanied by decreased MDA, Scr and BUN levels (p < 0.05 vs. untreated controls); bcl-2 mRNA and protein levels were increased in transplanted kidney from treated animals, while bax mRNA and protein levels were decreased (p < 0.05 vs. untreated controls). AI was significantly decreased in transplanted kidneys from treated animals relative to untreated controls (p < 0.05 vs. untreated controls).<bold>Conclusion: </bold>This study clearly demonstrates the protective effects on IRI after kidney transplantation, which may be explained by decreased lipid peroxidation and inhibition of apoptosis.
- Subjects
ISCHEMIA; REPERFUSION injury; KIDNEY transplantation; LABORATORY rats; SAPONINS; OXIDATIVE stress; APOPTOSIS; THERAPEUTICS; THERAPEUTIC use of plant extracts; ANIMAL experimentation; BIOLOGICAL models; COMPARATIVE studies; GENETIC techniques; GRAFT rejection; IMMUNOHISTOCHEMISTRY; RESEARCH methodology; MEDICAL cooperation; PLANTS; POLYMERASE chain reaction; PROTEINS; RATS; RESEARCH; RNA; SUPEROXIDE dismutase; MALONDIALDEHYDE; EVALUATION research; TREATMENT effectiveness; REVERSE transcriptase polymerase chain reaction; FREE radical scavengers
- Publication
Pediatric Surgery International, 2011, Vol 27, Issue 11, p1203
- ISSN
0179-0358
- Publication type
journal article
- DOI
10.1007/s00383-011-2935-z