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- Title
Comparison of PSA-specific CD8<sup>+</sup> CTL responses and antitumor immunity generated by plasmid DNA vaccines encoding PSA-HSP chimeric proteins.
- Authors
Pavlenko, Maxim; Roos, Anna-Karin; Leder, Christoph; Hansson, Lars-Olof; Kiessling, Rolf; Levitskaya, Elena; Pisa, Pavel
- Abstract
The ability of heat shock proteins (HSPs) to increase the potency of protein- and DNA-based vaccines has been previously reported. We have constructed several plasmid-based vectors encoding chimeric proteins containing prostate-specific antigen (PSA) fused toMycobacterium tuberculosishsp70,M. bovishsp65,Escherichia coliDnaK (hsp70), or human hsp70. Immunizing mice with these plasmids induced CD8+ cytotoxic T lymphocytes (CTLs) specific to human PSA and protected mice from a subsequent subcutaneous challenge with PSA-expressing tumors. We did not observe a significant difference either in the levels of PSA-specific CTLs or in protection against tumor challenge in mice immunized with plasmids expressing PSA-HSP chimeric proteins, as compared to mice receiving a conventional PSA-expressing DNA plasmid. Our data indicate that using HSPs as fusion partners for tumor-specific antigens does not always result in the enhancement of antigen-specific CTL responses when applied in the form of DNA vaccines.
- Subjects
PLASMIDS; HEAT shock proteins; TUMOR antigens; VACCINATION; DNA vaccines; TUMORS
- Publication
Cancer Immunology, Immunotherapy, 2004, Vol 53, Issue 12, p1085
- ISSN
0340-7004
- Publication type
Article
- DOI
10.1007/s00262-004-0559-z