We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
红平红球菌XH-1产海藻糖脂的培养条件优化及理化性质研究.
- Authors
李红芳; 梁生康; 段效辉; 赵梦娟; 董文迅; 韩晓红; 李俊峰
- Abstract
In order to reduce the fermentation cost and improve the trehalolipid production by Rhodococcus erythropolis XH-1, the optimal medium composition was determined by single factor tests and Box-Behnken tests. Results showed that the optimal medium formula were as follows: soybean cake powder 14.0 g/L, glycerin 36.2 g/L, yeast extract powder 0.2 g/L, KH2PO4 2.0 g/L, K2HPO4 2.0 g/L, NaCl 1.0 g/L, and KNO3 1.0 g/L. The optimum fermentation conditions were as follows: initial pH 9.0, fermentation temperature 30 ℃, shaking speed 150 r/min, liquid volume 45 ml/250 ml, inoculum 3%, and seed age 60 h. Under these optimal conditions, the trehalolipid yield of strain XH-1 (8.34 g/L) was 5.59 times that of before optimization. The results of physicochemical property analysis showed that the trehalolipid had higher surface activity and emulsifying ability, and had strong thermal stability (100 ℃, 2 h), acid-base stability (pH 2-10), and salt tolerance (0-20% NaCl). The research results provided theoretical support for the large-scale industrial production and application of trehalolipid.
- Subjects
RHODOCOCCUS erythropolis; YEAST extract; THERMAL stability; RESPONSE surfaces (Statistics); FERMENTATION
- Publication
China Brewing, 2023, Vol 42, Issue 6, p204
- ISSN
0254-5071
- Publication type
Article
- DOI
10.11882/j.issn.0254-5071.2023.06.033