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- Title
Isolation and characterization of novel cadmium-resistant Escherichia fergusonii ZSF-15 from industrial effluent for flocculant production and antioxidant enzyme activity.
- Authors
Zafar, Saima; Ashraf, Asma; Hayat, Sumreen; Siddique, Muhammad Hussnain; Waseem, Muhammad; Hassan, Mudassir; Qaisar, Hira; Muzammil, Saima
- Abstract
Cadmium (Cd) is a highly toxic metal that frequently contaminates our environment. In this study, the bioflocculant-producing, cadmium-resistant Escherichia fergusonii ZSF-15 was characterized from Paharang drain, Bawa Chak, Faisalabad, Pakistan. The Cd-resistant E. fergusonii was used to determine the bioflocculant production using yeast-peptone-glycerol medium (pH 6.5) supplemented with 50 mg L−1 of Cd. The culture was incubated for 3 days at 37 °C in a rotary shaker at 120 rpm. The fermentation broth was centrifuged at 4000 g for 10 min after the incubation period. The maximum flocculating activity by isolate ZSF-15 was found to be 71.4% after 48 h of incubation. According to the Fourier transform infrared spectroscopy analysis, the bioflocculant produced by strain ZSF-15 was comprised of typical polysaccharide and protein, i.e. hydroxyl, carboxyl, and amino groups. The strain ZSF-15 exhibited bioflocculant activity at range of pH (6–8) and temperature (35–50℃). Maximum flocculation activity (i.e. 71%) was observed at 47℃, whereas 63% flocculation production was observed at pH 8. In the present study, antioxidant enzyme profile of ZSF-15 was also evaluated under cadmium stress. A significant increase in antioxidant enzymes including superoxide dismutase (118%) and ascorbate peroxidase (28%) was observed, whereas contents of catalase (86%), glutathione transferase (13%), and peroxidase (8%) were decreased as compared to control.
- Subjects
FAISALABAD (Pakistan); FLOCCULANTS; INDUSTRIAL wastes; FOURIER transform infrared spectroscopy; CADMIUM poisoning; ESCHERICHIA; GLUTATHIONE transferase; ENZYMES
- Publication
Environmental Monitoring & Assessment, 2024, Vol 196, Issue 5, p1
- ISSN
0167-6369
- Publication type
Article
- DOI
10.1007/s10661-024-12545-x