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- Title
单细胞转录组测序溃疡性结肠炎脾虚湿蕴证患者的免疫细胞分子特征.
- Authors
张诗静; 黄卓健; 陶屈源; 侯江涛; 彭 彬; 罗泳欣; 李燕舞; 李辉标; 钟嘉敏; 陈新林
- Abstract
BACKGROUND: Immune-mediated tissue destruction is essential for the development and deterioration of inflammation in ulcerative colitis, but their roles remain unclear. Simultaneously, ulcerative colitis patients with spleen deficiency and dampness syndrome lack objective biomarkers. OBJECTIVE: To investigate the molecular characteristics of immune cells in ulcerative colitis patients with spleen deficiency and dampness based on single-cell RNA sequencing so as to provide a basis for its clinical diagnosis and treatment. METHODS: Colon tissues of two ulcerative colitis patients and two healthy controls were obtained. Single-cell RNA sequencing was performed. The following analyses were carried out for the single-cell RNA sequencing data: cluster analysis, dimensionality reduction analysis, cell type annotation, differential expression analysis and cell communication analysis. RESULTS AND CONCLUSION: (1) A total of 29 cell sub-types were identified according to clustering analysis in single cell transcriptome, which consisted of 9 cell types. (2) Compared with healthy controls, the ulcerative colitis patients with spleen deficiency and dampness syndrome had higher expression levels of CD4+ cytotoxic T cells, naive CD8+ T cells, germinal center, activated B cells, S100Ahigh B cells, NK_C2 cells and monocytes (P < 0.05). Compared with healthy controls, Tfh cells, NKT cells and NK cells decreased in patients with ulcerative colitis (P < 0.05). (3) Functional enrichment of differentially expressed genes showed that there were hyperinflammatory activation in immune cells and higher metabolic reprogramming of NK cells, plasma cells and helper T cells for ulcerative colitis patients. (4) Cell communication analysis identified 14 interactions among different cell types, mainly from monocytes and endothelial cells. (5) There are significant differences in molecular characteristics of immune cells between ulcerative colitis patients and healthy controls. These cell subsets may be related to the occurrence and development of ulcerative colitis, which may be a biomarker for ulcerative colitis patients with spleen deficiency and dampness syndrome.
- Subjects
B cells; T helper cells; ULCERATIVE colitis; CYTOTOXIC T cells; KILLER cells; CELL analysis; CELL communication
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2023, Vol 27, Issue 33, p5357
- ISSN
2095-4344
- Publication type
Article
- DOI
10.12307/2023.740