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- Title
中枢注射抗 Orexin 抗体对禁食大鼠摄食的抑制作用.
- Authors
刘诚; 张孟玲; 郭菲菲; 孙向荣; 徐珞
- Abstract
Objective: To discuss the effect of central injection of anti-Orexin antibody on feeding of fasted rats. Methods: The specificity of Orexin antibody was detected by immunohistochemistry and Western blot analysis. The distribution of Orexin positive neurons and positive nerve fibers in the brain was analyzed. The rats were sacrificed 24 h fasting and injected with anti-orexin antibody to calculate the effect on the food intake. Results: Western blot analysis shown that orexin antibody was able to detect the synthesis of orex-in-A. Immunohistochemistry analysis shown that Orexin-positive neurons existed in lateral hypothalamus and dome of the fornix. A large number of Orexin-positive nerve fibers were projected onto the arcuate nucleus, the periventricular hypothalamic nucleus, the paraventricular nucleus of the hypothalamus, the reuniens thalamic nucleus, the rhomboid thalamic nucleus, the paraventricular thalamic nucleus. the medial habenular nucleus and the stria medullaris thalamus, the central gray in the midbrain. the locus coeraleus. the nucleus ambiguus and the dorsal vagal complex. Compared with control IgG. intracerebroventricular injected 45 µg/10 µL anti-orexin antibody hi fasted rats inhibited the food mtakes (P<0.05). High dose of anti-orexin antibody could significantly inhibit the feeding of rats hi a dose-dependent maimer (P<0.05). Conclusions: Central injection of anti-orexin antibody in fasted rats inhibited food intake with a dose-dependent maimer.
- Publication
Progress in Modern Biomedicine, 2017, Vol 17, Issue 31, p6042
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2017.31.010