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- Title
Nogo-A expression in oligodendroglial tumors.
- Authors
Jung, Tae-Young; Jung, Shin; Lee, Kyung-Hwa; Cao, Van Thang; Jin, Shu-Guang; Moon, Kyung-Sub; Kim, In-Young; Kang, Sam-Suk; Kim, Hyung-Seok; Lee, Min-Cheol
- Abstract
Nogo-A belongs to the reticulon protein family and is expressed in the inner and outer loops of myelin sheaths of oligodendrocytes. We analyzed the patterns of Nogo-A expression in human gliomas in an effort to identify a useful marker for the characterization of oligodendroglial tumors. We determined the expression of Nogo-A in a panel of 58 astrocytic and oligodendroglial tumors using immunohistochemistry and compared the expression of Nogo-A with Olig-2, a recently identified marker for oligodendrogliomas. To localize Nogo-A expression, immunofluorescent staining was performed using other glial markers (MAP-2 and GFAP). We also confirmed the overexpression of the Nogo-A protein in 53 astrocytic and oligodendroglial tumors using Western blot analysis. Based on immunohistochemical analysis, Nogo-A and Olig-2 had specificity in the detection of oligodendroglial tumors from astrocytic tumors ( P = 0.001). The level of Nogo-A staining was highly correlated with Olig-2 ( P = 0.001). The sensitivity and specificity of Nogo-A for oligodendroglial tumors was 86.9% and 57.1%, respectively. Nogo-A expression overlapped that of other oligodendroglial markers, but with different patterns of expression. Western blot analysis revealed that Nogo-A is predominantly expressed in 85.7% of oligodendroglioma cells and 93.7% of anaplastic oligodendroglioma cells. Like other oligodendroglial markers, Nogo-A is highly expressed in oligodendroglial tumors; however, it does not serve as a definite marker specific for oligodendroglial tumors.
- Subjects
OLIGODENDROGLIA; PHYSIOLOGICAL effects of proteins; GENE expression; ASTROCYTOMAS; TUMOR markers; TUMORS
- Publication
Neuropathology, 2011, Vol 31, Issue 1, p11
- ISSN
0919-6544
- Publication type
Article
- DOI
10.1111/j.1440-1789.2010.01118.x