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- Title
Sir4 Deficiency Reverses Cell Senescence by Sub-Telomere Recombination.
- Authors
Liu, Jun; Hong, Xiaojing; Wang, Lihui; Liang, Chao-Ya; Liu, Jun-Ping; Gilley, David
- Abstract
Telomere shortening results in cellular senescence and the regulatory mechanisms remain unclear. Here, we report that the sub-telomere regions facilitate telomere lengthening by homologous recombination, thereby attenuating senescence in yeast Saccharomyces cerevisiae. The telomere protein complex Sir3/4 represses, whereas Rif1 promotes, the sub-telomere Y′ element recombination. Genetic disruption of SIR4 increases Y′ element abundance and rescues telomere-shortening-induced senescence in a Rad51-dependent manner, indicating a sub-telomere regulatory switch in regulating organismal senescence by DNA recombination. Inhibition of the sub-telomere recombination requires Sir4 binding to perinuclear protein Mps3 for telomere perinuclear localization and transcriptional repression of the telomeric repeat-containing RNA TERRA. Furthermore, Sir4 repression of Y′ element recombination is negatively regulated by Rif1 that mediates senescence-evasion induced by Sir4 deficiency. Thus, our results demonstrate a dual opposing control mechanism of sub-telomeric Y′ element recombination by Sir3/4 and Rif1 in the regulation of telomere shortening and cell senescence.
- Subjects
CELLULAR aging; TELOMERES; RECOMBINANT DNA; CARRIER proteins; SACCHAROMYCES cerevisiae; PROTEIN binding
- Publication
Cells (2073-4409), 2021, Vol 10, Issue 4, p778
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells10040778