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- Title
CD4<sup>+</sup> and CD8<sup>+</sup> skin-associated T lymphocytes in canine atopic dermatitis produce interleukin-13, interleukin-22 and interferon-γ and contain a CD25<sup>+</sup>FoxP3<sup>+</sup> subset.
- Authors
Jassies‐van der Lee, Annette; Rutten, Victor P. M. G.; Bruijn, Jet; Willemse, Ton; Broere, Femke
- Abstract
Background T Cells play a major role in the immunopathogenesis of canine atopic dermatitis ( cAD). However, the significance of cutaneous regulatory T cells (Tregs) and CD8+ T cells is currently unclear. Hypothesis/Objectives The study aimed to evaluate the presence and distribution of Tregs in cAD and healthy skin and to determine the cytokine production of cutaneous CD4+ and CD8+ T cells. Animals Biopsies were taken from four dogs with cAD (lesional and nonlesional skin) and four healthy control dogs. Methods Distribution patterns of T-cell subtypes in cAD lesional, nonlesional and control skin were evaluated by immunohistochemistry. Phenotypic characterization of T cells from skin explant cultures and enzymatic digestions was performed using flow cytometry. Cytokine production of sorted CD4+ and CD8+ explant-derived T cells was measured by RT- qPCR. Results Regulatory T cells phenotypically characterized by CD25+FoxP3+ were found in both CD4+ and CD8+ subsets of skin explant and digestion samples. The percentages of CD4+CD25+ cells that were FoxP3+ were similar in cAD and control skin. In atopic lesional and nonlesional explant samples, lower FoxP3+ percentages of CD8+CD25+ cells were seen compared with control skin. The presence of predominantly periadnexal CD25+FoxP3+ cells was confirmed by immunohistochemistry in lesional, nonlesional and control skin. The CD4+/CD8+ ratio was less than one in cAD skin with both skin explant and digestion methods. CD4+ and CD8+ T-cell subsets of lesional and nonlesional cAD skin were capable of producing interleukin-13, interleukin-22 and interferon-γ. Conclusions and clinical importance Both CD4+ and CD8+ T cells are likely to contribute to the immunopathogenesis of cAD through the production of interleukin-13, interleukin-22 and interferon-γ. In both subsets, functional analysis of FoxP3+ cells is essential to determine their role.
- Subjects
T cells; ATOPIC dermatitis; CANIDAE; INTERLEUKIN-13; CYTOKINES; IMMUNOHISTOCHEMISTRY; PHENOTYPES; DISEASES
- Publication
Veterinary Dermatology, 2014, Vol 25, Issue 5, p456
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/vde.12140