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- Title
Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers.
- Authors
Bolaric, S.; Barth, S.; Melchinger, A. E.; Posselt, U. K.
- Abstract
Perennial ryegrass (Lolium perenneL.) is the most important grass species for temperate grassland agriculture. The genetic relationship and distance among cultivars is largely unknown but of great interest for breeding programmes. The objectives of this study were to (i) investigate the molecular variation and structure of population cultivars, (ii) describe the relationship among cultivars in terms of the modified Rogers’ distance, and (iii) determine the minimum sample size required for characterization of cultivars ofL. perenneusing random amplified polymorphic DNA (RAPD) markers. A total of 22 ryegrass cultivars, mainly of European origin, were investigated with RAPD markers. The minimum sample size required for the characterization of cultivars was about 20 individuals per population. An analysis of molecular variance (amova) revealed a much larger genetic variation within cultivars (66%) than between them (34%).
- Subjects
LOLIUM perenne; RYEGRASSES; CULTIVARS; RAPD technique; PLANT genetics
- Publication
Plant Breeding, 2005, Vol 124, Issue 2, p161
- ISSN
0179-9541
- Publication type
Article
- DOI
10.1111/j.1439-0523.2004.01032.x