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- Title
T cells producing GM-CSF and IL-13 are enriched in the cerebrospinal fluid of relapsing MS patients.
- Authors
Ghezzi, Laura; Cantoni, Claudia; Cignarella, Francesca; Bollman, Bryan; Cross, Anne H; Salter, Amber; Galimberti, Daniela; Cella, Marina; Piccio, Laura
- Abstract
Background: Multiple sclerosis (MS) is a central nervous system (CNS) autoimmune demyelinating disease. Its pathogenesis involves humoral and cellular immunity, with production of pro- and anti-inflammatory cytokines by T cells. Objective: To analyze the cytokine profile of cerebrospinal fluid (CSF) T cells in patients with relapsing-remitting MS (RRMS) and non-inflammatory controls. Methods: T cell cytokine production was analyzed by flow cytometry in CSF samples collected from 34 untreated RRMS patients and 20 age-matched controls. Immunofluorescence studies were performed in spinal cord MS active lesions. Results: Percentages of CSF-derived IL-17A, IL-17A/IL-22, and IL-17A/GM-CSF producing T cells were significantly higher in RRMS patients compared to controls. Percentages of T cells producing IFN-γ were lower in RRMS patients compared to controls. Patients in relapse showed higher percentages of CD4+ T cells producing IL-13 and GM-CSF compared to patients in remission. We found a positive correlation between percentages of IL-13+ T cells and the Expanded Disability Status Scale (EDSS; ρ = 0.5; p < 0.05). Meningeal IL-13-producing T cells were detected in spinal cord MS active lesions. Conclusion: We observed differences in IL-17, IL-22, and IFN-γ production by CSF T cells in RRMS versus controls and a positive correlation between IL-13-producing T cells and EDSS in RRMS patients.
- Subjects
MYELIN sheath diseases; T cells; CEREBROSPINAL fluid; CENTRAL nervous system; DEMYELINATION; CELLULAR immunity
- Publication
Multiple Sclerosis Journal, 2020, Vol 26, Issue 10, p1172
- ISSN
1352-4585
- Publication type
Article
- DOI
10.1177/1352458519852092