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- Title
Expanding on Our Knowledge of Ecdysozoan Genomes: A Contiguous Assembly of the Meiofaunal Priapulan Tubiluchus corallicola.
- Authors
Lord, Arianna; Cunha, Tauana J; Medeiros, Bruno A S de; Sato, Shoyo; Khost, Danielle E; Sackton, Timothy B; Giribet, Gonzalo
- Abstract
Genomic data for priapulans are limited to a single species, restricting broad comparative analyses and thorough interrogation of questions spanning phylogenomics, ecdysozoan physiology, and development. To help fill this void, we present here a high-quality priapulan genome for the meiofaunal species Tubiluchus corallicola. Our assembly combines Nanopore and Illumina sequencing technologies and makes use of a whole-genome amplification, to generate enough DNA to sequence this small meiofaunal species. We generated a moderately contiguous assembly (2,547 scaffolds), with a high level of completeness (metazoan BUSCOs n = 954, single-copy complete = 89.6%, duplicated = 3.9%, fragmented = 3.5%, and missing = 3.0%). We then screened the genome for homologs of the Halloween genes, key genes implicated in the ecdysis (molting) pathway of arthropods, recovering a putative homolog of shadow. The presence of a shadow ortholog in two priapulan genomes suggests that the Halloween genes may not have evolved in a stepwise manner in Panarthropoda, as previously thought, but may have a deeper origin at the base of Ecdysozoa.
- Subjects
GENOMES; COMPARATIVE genomics; NUCLEOTIDE sequencing; ECDYSIS; DNA sequencing; PHYSIOLOGY; MOLTING
- Publication
Genome Biology & Evolution, 2023, Vol 15, Issue 6, p1
- ISSN
1759-6653
- Publication type
Article
- DOI
10.1093/gbe/evad103