We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid Takenouchi et al. IGFBP-2 and -3 in gingival crevicular fluid.
- Authors
Takenouchi, Y.; Ohshima, M.; Yamaguchi, Y.; Nishida, T.; Senda, N.; Idesawa, M.; Otsuka, K.; Ito, K.
- Abstract
Takenouchi Y, Ohshima M, Yamaguchi Y, Nishida T, Senda N, Idesawa M, Otsuka K, Ito K. Insulin-like growth factor-binding protein-2 and -3 in gingival crevicular fluid. J Periodont Res 2010; 45: 803-808. © 2010 John Wiley & Sons A/S Insulin-like growth factor-binding proteins (IGFBPs) are crucial regulators of insulin-like growth factor (IGF). They enhance or inhibit IGF functions, but also exhibit IGF-independent effects. In a previous study, we detected, qualitatively, IGFBP-2 and -3 in gingival crevicular fluid using a cytokine antibody array. Here we extended these results using an ELISA to determine the concentrations of IGFBP-2 and -3 in gingival crevicular fluid. In addition, we explored whether the expression of IGFBP-2 and IGFBP-3 correlates with periodontal disease severity. Gingival crevicular fluid samples from 92 sites of 12 patients affected with periodontal disease and from 100 sites of 19 healthy volunteers, were collected, divided into two groups and analyzed by ELISA for IGFBP-2 and -3 expression. The potential correlation among probing depth, gingival index and the concentrations of IGFBP-2 and -3 was analyzed. Positive correlations were observed between the concentration of IGFBP-2 and probing depth and gingival index, but not for IGFBP-3. The IGFBP-2 concentrations at bleeding on probing-positive sites and at sites with a probing depth of ≥ 4 mm were higher than at bleeding on probing-negative sites and at sites with a probing depth of ≤ 3 mm. These results indicate that IGFBP-2 is a potential novel marker for periodontal disease progression. As IGFBP-2 modulates bone metabolism and cell migration, IGFBP-2 in the gingival crevicular fluid may reflect periodontal disease activity.
- Subjects
JAPAN; PROTEIN analysis; STATISTICAL correlation; ENZYME-linked immunosorbent assay; GROWTH factors; PERIODONTAL disease; RESEARCH; U-statistics; SAMPLE size (Statistics); STATISTICAL significance
- Publication
Journal of Periodontal Research, 2010, Vol 45, Issue 6, p803
- ISSN
0022-3484
- Publication type
Article
- DOI
10.1111/j.1600-0765.2010.01309.x