We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Rhei Undulati Rhizoma attenuates memory decline and reduces amyloid-β induced neuritic dystrophy in 5xFAD mouse.
- Authors
Lee, Seungmin; Ju, In Gyoung; Eo, Hyeyoon; Kim, Jin Hee; Choi, Yujin; Oh, Myung Sook
- Abstract
Background: Alzheimer's disease (AD) is a common type of dementia characterized by amyloid-β (Aβ) accumulation, lysosomal dysfunction, and tau hyperphosphorylation, leading to neurite dystrophy and memory loss. This study aimed to investigate whether Rhei Undulati Rhizoma (RUR), which has been reported to have anti-neuroinflammatory effect, attenuates Aβ-induced memory impairment, neuritic dystrophy, and tau hyperphosphorylation, and to reveal its mode of action. Methods: Five-month-old 5xFAD mice received RUR (50 mg/kg) orally for 2 months. The Y-maze test was used to assess working memory. After behavioral testing, brain tissue was analyzed using thioflavin S staining, western blotting, and immunofluorescence staining to investigate the mode of action of RUR. To confirm whether RUR directly reduces Aβ aggregation, a thioflavin T assay and dot blot were performed after incubating Aβ with RUR. Results: RUR administration attenuated the Aβ-induced memory impairment in 5xFAD mice. Furthermore, decreased accumulation of Aβ was observed in the hippocampus of the RUR-treated 5xFAD group compare to the vehicle-treated 5xFAD group. Moreover, RUR reduced the dystrophic neurites (DNs) that accumulate impaired endolysosomal organelles around Aβ. In particular, RUR treatment downregulated the expression of β-site amyloid precursor protein cleaving enzyme 1 and the hyperphosphorylation of tau within DNs. Additionally, RUR directly suppressed the aggregation of Aβ, and eliminated Aβ oligomers in vitro. Conclusions: This study showed that RUR could attenuate Aβ-induced pathology and directly regulate the aggregation of Aβ. These results suggest that RUR could be an efficient material for AD treatment through Aβ regulation.
- Subjects
PHYTOTHERAPY; TAU proteins; POLYMERS; IN vitro studies; ALZHEIMER'S disease; DATA analysis; T-test (Statistics); RESEARCH funding; ENZYME-linked immunosorbent assay; FLUORESCENT antibody technique; DESCRIPTIVE statistics; PLANT extracts; MICE; ANIMAL experimentation; WESTERN immunoblotting; ONE-way analysis of variance; STATISTICS; STAINS &; staining (Microscopy); HIPPOCAMPUS (Brain); DATA analysis software; MEMORY disorders; AMYLOID beta-protein precursor
- Publication
Chinese Medicine, 2024, Vol 19, Issue 1, p1
- ISSN
1749-8546
- Publication type
Article
- DOI
10.1186/s13020-024-00966-2