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- Title
ROLE OF SELENIUM ON MPP+ NEUROTOXICITY IN SH-SY5Y NEUROBLASTOMA CELLS: FOCUS ON TRPV1 CHANNELS.
- Authors
Öz, Ahmi; Çelik, Ömer; Nazıroğlu, Mustafa
- Abstract
OBJECTIVES: Selenium, co-factor of most powerful intracellular antioxidant glutathione peroxidase enzyme, is one of trace elements that found in the body. The SH-SY5Y human neuroblastoma cell line is frequently used for in vitro model of Parkinson's disease. The 1-methyl-4-phenylpyridinium (MPP+) is a toxic metabolite and breakdown of mitochondrial complex-1 by its activity is known that induce Parkinson's pathology in SH-SY5Y cell line through an increasing the cellular redox level and decreasing cell viability. The TRPV1 channels are oxidative stress-sensitive and calcium-permeable non-selective cation channels that widely expressed in neuronal cells. Hence, we aimed to investigate that the role of selenium supplementation and TRPV1 channel activity on MPP+ induced neurotoxicity in SH-SY5Y cells. MATERIAL&METHODS: For this aim, we grouped cells as 1- Control, 2- Selenium, 3- MPP and 4- MPP + Selenium. After all incubations, different cellular functions such as Wright-Giemsa staining for assessment of apoptosis and calcium signaling (fura-2) for TRPV1 mediated calcium influx were evaluated in study groups. RESULTS: Intracellular calcium concentration was statistically lower in MPP group comparing to control. However, selenium treatment alters that level to control. It is determined that low concentration of selenium contributes to cell viability and it modulates MPP+ induced apoptosis. CONCLUSIONS: We observed novel effects of selenium on the regulation of calcium signaling via TRPV1. Selenium treatment can be useful for the inhibition of neurodegeneration induced by MPP+. Further studies are needed to illuminate role of TRPV1 channels in molecular mechanisms of MPP induced apoptosis in neuronal cells.
- Subjects
TRPV cation channels; SELENIUM; TRP channels; NEUROTOXICOLOGY; NEUROBLASTOMA; PARKINSON'S disease; GLUTATHIONE peroxidase
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2019, Vol 44, p60
- ISSN
0250-4685
- Publication type
Article