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- Title
Intermittent flow increases endotoxin-induced adhesion of human erythrocytes to vascular endothelial cells.
- Authors
Eichelbrönner, Otto; Sibbald, William J.; Chin-Yee, Ian H.; Eichelbrönner, Otto
- Abstract
<bold>Objective: </bold>To investigate the effects of different conditions of flow on endotoxin induced adhesion of human red blood cells (RBC) to human umbilical vein endothelial cells (HUVEC).<bold>Design and Setting: </bold>Prospective, randomized, controlled in vitro study in a university-affiliated cell biology laboratory. SUBJECTS. Human erythrocytes, human vascular endothelial cells.<bold>Interventions: </bold>Superfusion of HUVEC monolayers with human erythrocytes incubated with either saline (CON) or endotoxin (ETX) with different flow pattern (basic flow rates of 0.65 or 1.3 ml/min; intermittent flow, IMF). The CON/0.6, CON/1.3, CON-IMF/1.3 ( n=7/group) groups served as control, and in test groups ETX/0.6, ETX/1.3, ETX-IMF/0.6, and ETX-IMF/1.3 ( n=7/group) both RBC and HUVECs were incubated with ETX and flow pattern and rates varied. In the IMF experiments flow rates of 0.65 and 1.3 ml/min were combined with stop-and-go flow pattern.<bold>Measurements and Results: </bold>At continuous flow of 0.65 ml/min erythrocyte adhesion was 61+/-5 cells/mm(2) in CON and 172+/-25 cells/mm(2) after ETX. When flow rate was increased to 1.3 ml/min, adhesion decreased to 27+/-4 cells/mm(2) in CON and 93+/-18 cells/mm(2) after ETX. IMF conditions had no effect on RBC adhesion of naive RBC but increased the number of adhesive erythrocytes after incubation with ETX both at 0.65 ml/min (287+/-33 cells/mm(2)) and at 1.3 ml/min (148+/-13 cells/mm(2)).<bold>Conclusions: </bold>RBC adhesion to vascular endothelium is affected by rate and pattern of blood flow. Higher flow rates or shear forces reduce RBC adhesion while stop-and-go flow pattern favored adhesion of ETX-treated erythrocytes to HUVECs. These findings suggest that altered RBCs interact with altered flow patterns potentially contributing to the microcirculatory injury observed in sepsis.
- Subjects
ERYTHROCYTES; BLOOD cells; ENDOTOXINS; BLOOD vessels; ENDOTHELINS; VASCULAR endothelium; CELL culture; CELL physiology; COMPARATIVE studies; ENDOTHELIUM; RESEARCH methodology; MEDICAL cooperation; RESEARCH; EVALUATION research; UMBILICAL veins
- Publication
Intensive Care Medicine, 2003, Vol 29, Issue 5, p709
- ISSN
0342-4642
- Publication type
journal article
- DOI
10.1007/s00134-003-1698-y