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- Title
Monoreassortant Rotaviruses of Multiple G Types Are Differentially Neutralized by Sera From Infants Vaccinated With ROTARIX and RotaTeq.
- Authors
Diller, Julia R; Carter, Maximilian H; Kanai, Yuta; Sanchez, Shania V; Kobayashi, Takeshi; Ogden, Kristen M
- Abstract
<bold>Background: </bold>Rotavirus is a leading cause of pediatric diarrheal mortality. The rotavirus outer capsid consists of VP7 and VP4 proteins, which, respectively, determine viral G and P type and are primary targets of neutralizing antibodies.<bold>Methods: </bold>To elucidate VP7-specific neutralizing antibody responses, we engineered monoreassortant rotaviruses each containing a human VP7 segment from a sequenced clinical specimen or a vaccine strain in an identical genetic background. We quantified replication and neutralization of engineered viruses using sera from infants vaccinated with monovalent ROTARIX or multivalent RotaTeq vaccines.<bold>Results: </bold>Immunization with RotaTeq induced broader neutralizing antibody responses than ROTARIX. Inclusion of a single dose of RotaTeq in the schedule enhanced G-type neutralization breadth of vaccinated infant sera. Cell type-specific differences in infectivity, replication, and neutralization were detected for some monoreassortant viruses.<bold>Conclusions: </bold>These findings suggest that rotavirus VP7, independent of VP4, can contribute to cell tropism and the breadth of vaccine-elicited neutralizing antibody responses.
- Subjects
VACCINATION; ROTAVIRUSES; ANTIBODY formation; INFANTS; IMMUNIZATION
- Publication
Journal of Infectious Diseases, 2021, Vol 224, Issue 10, p1720
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiab479