We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
F-spondin.
- Authors
Schubert, David; Klar, Avihu; Park, Minkyu; Dargusch, Richard; Fischer, Wolfgang H.
- Abstract
Cells in the developing nervous system secrete a large number of proteins that regulate the migration and differentiation of their neighbors. It is shown here that a clonal central nervous system cell line secretes a protein that causes both a rat hippocampal progenitor cell line and primary cortical neural cells to differentiate into cells with the morphological and biochemical features of neurons. This protein was identified as F-spondin. Analysis of F-spondin isoforms secreted from transfected cells shows that the core protein without the thrombospondin type 1 repeats is sufficient to promote neuronal differentiation when adsorbed to a surface. F-spondin can also inhibit neurite outgrowth while allowing the expression of nerve-specific proteins when present in a soluble form at high concentrations. Therefore, F-spondin can alter cell differentiation in multiple ways, depending upon its concentration and distribution between substrate-attached and soluble forms.
- Subjects
CELLS; NERVOUS system; PROTEINS; CELL migration; CELL differentiation; CENTRAL nervous system; CELL lines
- Publication
Journal of Neurochemistry, 2006, Vol 96, Issue 2, p444
- ISSN
0022-3042
- Publication type
Article
- DOI
10.1111/j.1471-4159.2005.03563.x