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- Title
The Effect of c-Fos Demethylation on Sodium Fluoride-induced Apoptosis in L-02 Cells.
- Authors
Niu, Qiang; Liu, HongLiang; Guan, ZhiZhong; Zeng, Qiang; Guo, ShuXia; He, Ping; Guo, LiJuan; Gao, Ping; Xu, BaYi; Xu, ZhiXia; Xia, Tao; Wang, AiGuo
- Abstract
To investigate the effects of sodium fluoride (NaF) on apoptosis, c-Fos mRNA and protein expression levels, and methylation status as well as Dnmt1, Dnmt3a, and Dnmt3b mRNA expression levels in human embryo hepatocyte (L-02) which were exposed to different concentrations of NaF (0, 20, 40, and 80 mg/l) for 24 h in vitro. Results showed that the percentage of apoptosis and c-Fos mRNA and protein expression levels in 40 and 80 mg/l NaF-treated groups were higher than those in the control group ( P < 0.05). Further, Dnmt1 mRNA expression level was significantly decreased in the 80 mg/l NaF-treated groups compared to the control group ( P < 0.05); Dnmt3a and Dnmt3b mRNA expression levels were significantly decreased in 40 and 80 mg/l NaF-treated groups compared to the control group ( P < 0.05). c-Fos methylation levels, according to the bisulfite sequencing results, were decreased in 20, 40, and 80 mg/l NaF-treated groups against the control group. These results suggest that NaF could induce apoptosis and upregulate mRNA and protein expression level of c-Fos as well as decrease mRNA expression levels of Dnmt1, Dnmt3a, and Dnmt3b in L-02 cells. The decrease in c-Fos methylation levels might be involved in the early phase of apoptosis induced by NaF in L-02 cells.
- Subjects
SODIUM fluoride; METHYLATION; LIVER cells; APOPTOSIS; EMBRYOLOGY
- Publication
Biological Trace Element Research, 2012, Vol 149, Issue 1, p102
- ISSN
0163-4984
- Publication type
Article
- DOI
10.1007/s12011-012-9392-y