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- Title
Identification and Characterization of a Novel Methionine Sulfoxide Reductase B Gene (AccMsrB) from Apis cerana cerana (Hymenoptera: Apidae).
- Authors
FENG LIU; ZHIHONG GONG; WEIXING ZHANG; YING WANG; LANTING MA; HONGFANG WANG; XINGQI GUO; BAOHUA XU
- Abstract
Apis cerana cerana F., designated AccMsrB. The full cDNA of AccMsrB was 757bp with an open reading frame (ORF) of 414 bp, and the predicted translation product was a 137 amino acid polypeptide with an estimated molecular mass of 15.5kDa and an isoelectric point of 7.77. Multiple sequence alignment revealed that AccMsrB shares high identity with other known MsrBs (Apis florea F., Apis mellifera L., Apis dorsata F., and Bombus terrestris L.) and contains conservative Cys residues. Quantitative real-time polymerase chain reaction showed AccMsrB to be highly expressed in the epidermis of adult workers, reaching high levels in first-instar larvae, prepupae, and 15-d-old adults. Furthermore, the expression of AccMsrB was upregulated by various oxidative stresses, including 4, 16, 25, and 42°C, ultraviolet light (30mj/cm2), H2O2, CdCl2, HgCl2, paraquat, imidacloprid, and cyhalothrin. However, AccMsrB was downregulated when exposed to phoxime. These results indicate that AccMsrB might respond to various environmental stresses and protect against reactive oxygen species.
- Subjects
METHIONINE sulfoxide reductase; APIS cerana; HYMENOPTERA; OPEN reading frames (Genetics); INSECT genetics; GENE expression; REACTIVE oxygen species; OXIDATIVE stress
- Publication
Annals of the Entomological Society of America, 2015, Vol 108, Issue 4, p575
- ISSN
0013-8746
- Publication type
Article
- DOI
10.1093/aesa/sav042