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- Title
In vitro adventitious shoot formation from embryonic and cotyledonary tissues of Pinus brutia Ten.
- Authors
Abdullah, Anwar; Yeoman, Michael; Grace, John
- Abstract
Adventitious buds were induced when isolated whole embryos, and excised cotyledons from treated seeds of Calabrian pine ( Pinus brutia Ten.) were cultured on a cytokinin-supplemented medium. The adventitious buds formed directly from the cotyledons. The highest number of bud primordia were formed, with both embryos and excised cotyledons, after 6 weeks on a BAP - supplemented Schenk and Hildebrandt medium under a 16 h photoperiod. The buds, when separated and maintained individually on a full-strength medium without growth regulators, developed into well-formed shoots within 4 weeks. The average number of harvested shoots obtained (>1 cm in height) per seed over 24 weeks was 55; however, a maximum number of 152 shoots was obtained from one individual over the same period. The shoot forming capacity of the meristematic tissue was not lost after seven harvests.
- Publication
Plant Cell, Tissue & Organ Culture, 1985, Vol 5, Issue 1, p35
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/BF00033567