We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Specific detection and semi-quantitative analysis of TRPC4 protein expression by antibodies.
- Authors
Flockerzi, Veit; Jung, Christine; Aberle, Thomas; Meissner, Marcel; Freichel, Marc; Philipp, Stephan E.; Nastainczyk, Wolfgang; Maurer, Patrick; Zimmermann, Richard
- Abstract
In mouse tissues two variants of the transient receptor potential (canonical) (TRPC) 4 protein are expressed: the “full-length” TRPC4 protein and a slightly smaller variant, called TRPC4Δ761-864, which lacks 84 amino acid residues. Although the presence of mRNA encoding the TRPC4 protein in mammalian cells and the detection of the heterologously expressed TRPC4 protein by Western blot analysis have been reported, the unequivocal detection of endogenous TRPC4 proteins has proven difficult. In the present study we compared polyclonal antibodies for the detection of TRPC4 proteins in mouse tissues and monitored their specificity and reliability by analysing corresponding tissues from TRPC4-deficient mice. In addition we introduced a procedure that allows us to estimate the amount of TRPC4 protein expressed in a single cell. Using this technique it appears that the amount of TRPC4 protein expressed stably in HEK 293 cells is at least fourfold higher than the amount of TRPC4 protein expressed endogenously in the bovine adrenocortical cell line SBAC.
- Subjects
TRP channels; ION channels; MEMBRANE proteins; PROTEINS; IMMUNOGLOBULINS
- Publication
Pflügers Archiv: European Journal of Physiology, 2005, Vol 451, Issue 1, p81
- ISSN
0031-6768
- Publication type
Article
- DOI
10.1007/s00424-005-1443-1