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- Title
Genome-Wide Identification of Ascorbate Peroxidase Gene Family in Two Contrasting Barley (Hordeum vulgare L.) Cultivars in Response to Abiotic Stresses.
- Authors
Saidi, A.; Hajibarat, Z.; Ghazvini, H.
- Abstract
Reactive oxygen species, as main molecules, participate in the adaptation process of plants under environmental stimuli. Excessive oxidation and reduction of cellular components are equally harmful, so maintaining redox homeostasis is critical. The comprehensive analysis of HvAPX genes in barley has not yet been described. In this study, eight ascorbate peroxidase (APX) gene families in barley were identified. The barley APX families were characterized for phylogenetic tree, conserved motifs, gene ontology, correlation of traits with gene expression, prediction of cis-elements, and gene expression in APX under abiotic stress conditions. In addition, the analysis of enzymes activities was performed on two contrasting Iranian barley cultivars namely Sahra (drought tolerant) and Nobahar (drought susceptible) under abiotic stress (PEG, heat, ABA, and salt) conditions. The Sahra, an early maturing cultivar with a longer awn, has a higher drought resistance than Nobahar cultivar. Gene expression analysis revealed that 8 HvAPX genes were accumulated in the leaf and root tissues at 24 and 48 hours after being subjected to abiotic stresses. Furthermore, the gene expression analysis of the HvAPX genes revealed that gene expression was up/down regulated in response to PEG-induced drought stress, ABA, salt, and heat stresses in the leaf and root tissues. The phylogenetic analysis of the HvAPX proteins sequences in barley were grouped into three clusters. The HvAPX7 and HvAPX8 genes had the highest number of cis-elements in their promoter regions, indicating that they might be stimulated by a plethora of environmental stresses. The HvAPX genes had GT1-motif, STRE, CAAT-box, MYB, and MYC in their promoter regions, playing a key role in response to abiotic stresses. Our findings provide new insights into APX genes and provide a basis for next investigations of APX genes in plant improvement (breeding) programs.
- Subjects
CULTIVARS; BARLEY; GENE families; ABIOTIC stress; HORDEUM; PEROXIDASE; PLANT genes; GENE expression
- Publication
Russian Journal of Plant Physiology, 2024, Vol 71, Issue 1, p1
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S1021443723602288