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- Title
Development of a new evaluation system for the selection of probiotic lactic acid bacteria (LAB) with specific adhesion to human blood type A-antigen of intestinal mucosa.
- Authors
Uchida, H.; Kinoshita, H.; Kawai, Y.; Kitazawa, H.; Miura, K.; Shiiba, K.; Horii, A.; Kimura, K.; Taketomo, N.; Oda, M.; Yajima, T.; Saito, T.
- Abstract
A new evaluation system for selecting probiotic lactic acid bacteria (LAB) with activity for specific adhesion to human colonic mucin by recognizing differences in ABO-blood type was established. Sixteen strains that showed strong adhesion to human blood type-A antigen [GalNAc-α-1-3 (Fuc-α-1-2) Gal-] of intestinal mucosa were selected from 237 probiotic strains with a biosensor BIACORE, by surface plasmon resonance (SPR). Similarly, sixteen and eleven strains were selected with strong activity to human B-antigen [Gal-α-1-3 (Fuc- α-1-2) Gal-] and H-antigen [Fuc-α-1-2 Gal-], respectively. Because there is a lot of population of human blood type-A in Japan (40%), we experimented the LAB recognizing human A-antigen for further evaluation. The adherence of the SLPs (surface layer proteins) prepared from the selected strains towards human A-antigen was reevaluated by BIACORE and the L. brevis strains revealed strong adhesion to the A-antigen. The SLP from L. brevis strain OLL2772 showed a single band at ca. 48 kDa using SDS-PAGE analysis and it had a very high adherence to the human A-antigen as shown using an anti-A lectin blocking technique. A partial N-terminal sequence of the band showed high homology to a S-layer protein of L. brevis ATCC 8287T . There are few reports of LAB recognizing human blood type antigens in comparison to those showing the adhesion of LAB to the human intestine. The new assay system will be useful for the selection of probiotic candidates in future construction of functional foods including yogurt.
- Subjects
JAPAN; INTESTINAL mucosa; LACTIC acid bacteria; PROBIOTICS; BLOOD groups; SURFACE plasmon resonance; ADHESION
- Publication
Journal of Animal Science, 2006, Vol 84, p278
- ISSN
0021-8812
- Publication type
Article