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- Title
A role for Yip 1p in COPII Vesicle biogenesis.
- Authors
Heidtman, Matthew; Chen, Catherine Z.; Collins, Ruth N.; Barlowe, Charles
- Abstract
Yeast Ypt1p-interacting protein (Yip1p) belongs to a conserved family of transmembrane proteins that interact with Rab GTPases. We encountered Yip1p as a constituent of ER-derived transport vesicles, leading us to hypothesize a direct role for this protein in transport through the early secretory pathway. Using a cell-free assay that recapitulates protein transport from the ER to the Golgi complex, we find that affinity-purified antibodies directed against the hydrophilic amino terminus of Yip1p potently inhibit transport. Surprisingly, inhibition is specific to the COPII-dependent budding stage. In support of this in vitro observation, strains bearing the temperature-sensitive yip1-4 allele accumulate ER membranes at a nonpermissive temperature, with no apparent accumulation of vesicle intermediates. Genetic interaction analyses of the yip1-4 mutation corroborate a function in ER budding. Finally, ordering experiments show that preincubation of ER membranes with COPII proteins decreases sensitivity to anti-Yip1p antibodies, indicating an early requirement for Yip1p in vesicle formation. We propose that Yip1p has a previously unappreciated role in COPII vesicle biogenesis.
- Subjects
MEMBRANE proteins; ORIGIN of life; IMMUNOGLOBULINS; CELLS
- Publication
Journal of Cell Biology, 2003, Vol 163, Issue 1, p57
- ISSN
0021-9525
- Publication type
Article
- DOI
10.1083/jcb.200306118