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- Title
High-Content Imaging-Based Assay for SARS-CoV-2-Neutralizing Antibodies.
- Authors
Rocha, Vinícius Pinto Costa; Machado, Bruna Aparecida Souza; Quadros, Helenita Costa; Fernandes, Antônio Márcio Santana; Fiuza, Bianca Sampaio Dotto; Meira, Cássio Santana; da Silva, Vitória Torres Barbosa; Evangelista, Afrânio Ferreira; Fonseca, Larissa Moraes dos Santos; Badaró, Roberto José da Silva; Soares, Milena Botelho Pereira
- Abstract
The COVID-19 pandemic and the consequent emergence of new SARS-CoV-2 variants of concern necessitates the determination of populational serum potency against the virus. Here, we standardized and validated an imaging-based method to quantify neutralizing antibodies against lentiviral particles expressing the spike glycoprotein (pseudovirus). This method was found to efficiently quantify viral titers based on ZsGreen-positive cells and detect changes in human serum neutralization capacity induced by vaccination with up to two doses of CoronaVac, Comirnaty, or Covishield vaccines. The imaging-based protocol was also used to quantify serum potency against pseudoviruses expressing spikes from Delta, Omicron BA.1.1.529, and BA.4/5. Our results revealed increases in serum potency after one and two doses of the vaccines evaluated and demonstrated that Delta and Omicron variants escape from antibody neutralization. The method presented herein represents a valuable tool for the screening of antibodies and small molecules capable of blocking viral entry and could be used to evaluate humoral immunity developed by different populations and for vaccine development.
- Subjects
SARS-CoV-2 Omicron variant; SARS-CoV-2 Delta variant; IMMUNOGLOBULINS; SARS-CoV-2; SMALL molecules
- Publication
Vaccines, 2024, Vol 12, Issue 3, p236
- ISSN
2076-393X
- Publication type
Article
- DOI
10.3390/vaccines12030236