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- Title
The Role of Ferroptosis in the Damage of Human Proximal Tubule Epithelial Cells Caused by Perfluorooctane Sulfonate.
- Authors
Wang, Pingwei; Liu, Dongge; Yan, Shuqi; Liang, Yujun; Cui, Jiajing; Guo, Li; Ren, Shuping; Chen, Peng
- Abstract
Perfluorooctane sulfonate (PFOS) is a typical persistent organic pollutant and environmental endocrine disruptor that has been shown to be associated with the development of many diseases; it poses a considerable threat to the ecological environment and to human health. PFOS is known to cause damage to renal cells; however, studies of PFOS-induced ferroptosis in cells have not been reported. We used the CCK-8 method to detect cell viability, flow cytometry and immunofluorescence methods to detect ROS levels and Western blot to detect ferroptosis, endoplasmic reticulum stress, antioxidant and apoptosis-related proteins. In our study, we found that PFOS could induce the onset of ferroptosis in HK-2 cells with decreased GPx4 expression and elevated ACSL4 and FTH1 expression, which are hallmarks for the development of ferroptosis. In addition, PFOS-induced ferroptosis in HK-2 cells could be reversed by Fer-1. We also found that endoplasmic reticulum stress and its mediated apoptotic mechanism and P53-mediated antioxidant mechanism are involved in the toxic damage of cells by PFOS. In this paper, we demonstrated for the first time that PFOS can induce ferroptosis in HK-2 cells. In addition, we preliminarily explored other mechanisms of cytotoxic damage by PFOS, which provides a new idea to study the toxicity of PFOS as well as the damage to the kidney and its mechanism.
- Subjects
ENDOPLASMIC reticulum; PERFLUOROOCTANE sulfonate; EPITHELIAL cells; PERSISTENT pollutants; POLLUTANTS; ENDOCRINE disruptors
- Publication
Toxics, 2022, Vol 10, Issue 8, p436
- ISSN
2305-6304
- Publication type
Article
- DOI
10.3390/toxics10080436