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- Title
Inducing Neuronal Injury on NSC-34 Motor Neuron-Like Cells by Kainate Receptor Excitotoxicity.
- Authors
Aziz, Nor Alia Abd; Daud, Muhammad Fauzi; Lokanathan, Yogeswaran; Yazid, Muhammad Da'in; Idris, Jalilah
- Abstract
Introduction: Glutamate toxicity is one of the mechanisms involved in motor neuron degeneration pathogenesis, such as amyotrophic lateral sclerosis and spinal cord injury. The discoveries on glutamate toxicity have mainly focused on ionotropic NMDA and AMPA receptors. However, little is known about neuronal degeneration caused by excitotoxicity of the kainate receptor. A hybrid cell line, NSC-34 is the fusion of mouse spinal cord neurons with neuroblastoma cells. It is commonly used to study the pathophysiology of motor neurons. Therefore, the aim of this study is to investigate the role of kainate receptors in mediating excitotoxicity in motor neuron-like cells, NSC-34. Methods: NSC-34 cells were differentiated by serum deprivation and treatment with all-trans retinoic acid. Differentiated NSC-34 cells were exposed to kainate receptor agonist, kainic acid at various concentrations. Apoptotic activity was assessed using Annexin V staining and propidium iodide. Expression of the apoptotic and injury markers were measured using quantitative polymerase chain reaction (qPCR). Results: Findings indicate that differentiated NSC-34 cells responded to high concentrations of kainic acid. There is an increased number of cell death as the concentration of kainic acid increased. Conclusion: High concentrations of kainic acid trigger excitotoxicity of kainate receptor. Therefore, this suggests that kainate-induced excitotoxicity has a potential role in motor neuron degeneration.
- Subjects
TRETINOIN; GLUTAMATE receptors; CELL receptors; MOTOR neurons; KAINIC acid; AMYOTROPHIC lateral sclerosis; NEURODEGENERATION
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p11
- ISSN
1675-8544
- Publication type
Article