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- Title
Analysis of the formalin-fixed paraffin-embedded tissue proteome: pitfalls, challenges, and future prospectives.
- Authors
Maes, Evelyne; Broeckx, Valérie; Mertens, Inge; Sagaert, Xavier; Prenen, Hans; Landuyt, Bart; Schoofs, Liliane
- Abstract
Formalin-fixed paraffin-embedded (FFPE) tissues are a real treasure for retrospective analysis considering the amount of samples present in hospital archives, combined with pathological, clinical, and outcome information available for every sample. Although unlocking the proteome of these tissues is still a challenge, new approaches are being developed. In this review, we summarize the different mass spectrometry platforms that are used in human clinical studies to unravel the FFPE proteome. The different ways of extracting crosslinked proteins and the analytical strategies are pointed out. Also, the pitfalls and challenges concerning the quality of FFPE proteomic approaches are depicted. We also evaluated the potential of these analytical methods for future clinical FFPE proteomics applications.
- Subjects
FORMALDEHYDE; PARAFFIN wax; PROTEOMICS; PATHOLOGY; RETROSPECTIVE studies; HEALTH outcome assessment; PROTEIN crosslinking
- Publication
Amino Acids, 2013, Vol 45, Issue 2, p205
- ISSN
0939-4451
- Publication type
Article
- DOI
10.1007/s00726-013-1494-0