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- Title
Induction of Human T-cell and Cytokine Responses Following Vaccination with a Novel Influenza Vaccine.
- Authors
Skibinski, David A. G.; Jones, Leigh Ann; Zhu, Yuan O.; Xue, Lin Wu; Au, Bijin; Lee, Bernett; Naim, Ahmad Nazri Mohamed; Lee, Audrey; Kaliaperumal, Nivashini; Low, Jenny G. H.; Lee, Lawrence S.; Poidinger, Michael; Saudan, Philippe; Bachmann, Martin; Ooi, Eng Eong; Hanson, Brendon J.; Novotny-Diermayr, Veronica; Matter, Alex; Fairhurst, Anna-Marie; Hibberd, Martin L.
- Abstract
Cell mediated immunity plays a vital role in defense against influenza infection in humans. Less is known about the role of vaccine-induced cell mediated immunity and the cytokine responses elicited. We measured CD4+ and CD8+ T-cell reactivity in human subjects following vaccination with licensed trivalent influenza vaccine and a novel virus-like particle based vaccine. We detected influenza-specific CD4+ T-cell responses following vaccination with the licensed trivalent influenza vaccine and found that these correlated with antibody measurements. Administration of the novel virus-like particle based vaccine elicited influenza-specific CD4+ and CD8+ T-cell responses and the induction of the cytokines IFN-γ, IL-17A, IL17F, IL-5, IL-13, IL-9, IL-10 and IL-21. Pre-existing cytokine responses influenced the profile of the cytokine response elicited by vaccination. In a subset of individuals the VLP vaccine changed pre-vaccination production of type 2 cytokines such as IL-5 and IL-13 to a post-vaccination type 1 cytokine signature characterized by IFN-γ. A transcriptional signature to vaccination was found to correlate with antibody titer, IFN-γ production by T-cells and expression of a putative RNA helicase, DDX17, on the surface of immune cells.
- Publication
Scientific Reports, 2018, Vol 8, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-018-36703-7