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- Title
Phylogenetic analysis of enterohemorrhagic Escherichia coli O157, Germany, 1987-2008.
- Authors
Jenke C; Harmsen D; Weniger T; Rothganger J; Hyytia-Trees E; Bielaszewska M; Karch H; Mellmann A; Jenke, Christian; Harmsen, Dag; Weniger, Thomas; Rothganger, Jorg; Hyytia-Trees, Eija; Bielaszewska, Martina; Karch, Helge; Mellmann, Alexander
- Abstract
Multilocus variable number tandem repeat analysis (MLVA) is a subtyping technique for characterizing human pathogenic bacteria such as enterohemorrhagic Escherichia coli (EHEC) O157. We determined the phylogeny of 202 epidemiologically unrelated EHEC O157:H7/H- clinical isolates through 8 MLVA loci obtained in Germany during 1987-2008. Biodiversity in the loci ranged from 0.66 to 0.90. Four of 8 loci showed null alleles and a frequency < or =44.1%. These loci were distributed among 48.5% of all strains. Overall, 141 MLVA profiles were identified. Phylogenetic analysis assigned 67.3% of the strains to 19 MLVA clusters. Specific MLVA profiles with an evolutionary persistence were identified, particularly within sorbitol-fermenting EHEC O157:H-.These pathogens belonged to the same MLVA cluster. Our findings indicate successful persistence of this clone.
- Publication
Emerging Infectious Diseases, 2010, Vol 16, Issue 4, p610
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid1604.091361