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- Title
Bergamottin Inhibits Adipogenesis in 3T3-L1 Cells and Weight Regulation in Diet-Induced Obese Mice.
- Authors
Ko, Jeong-Hyeon; Nam, Dongwoo; Um, Jae-Young; Jung, Sang Hoon; Ahn, Kwang Seok
- Abstract
Obesity is a serious and increasing health problem worldwide, and the inhibition of adipogenesis is considered to be a potential therapeutic target for it. Bergamottin (BGM), a component of grapefruit juice, has been reported to regulate lipolysis. However, the physiological role of BGM in obesity has not been evaluated so far. In the present study, we investigated the effects of BGM on obesity in 3T3-L1 cells and in mice fed a high-fat diet (HFD). BGM inhibited adipogenic differentiation of 3T3-L1 cells along with a significant decrease in the lipid content by downregulating the expression of two critical adipogenic factors, CCAAT enhancer-binding protein-alpha (C/EBP) and peroxisome proliferator activated receptor-gamma (PPAR). The expressions of target proteins such as adipocyte fatty acid-binding protein (aP2), adiponectin, and resistin were also decreased by BGM. It activated AMP-activated protein kinase (AMPK) by increasing phosphorylation of AMPK and the downstream target acetyl-CoA carboxylase (ACC), indicating that BGM exerted its antiadipogenic effect through AMPK activation. In the HFD-induced obese mouse model, BGM administration significantly reduced the weight and sizes of white adipose tissue as well as the weight gain of mice fed HFD. Moreover, UCP1 and PGC1 expressions, well-known as brown adipocyte marker genes, were higher in the BGM-treated HFD mice than that in the HFD-induced obese mice. This study suggests that BGM suppress adipogenesis by AMPK activation in vitro and reduces body weight in vivo.
- Subjects
OBESITY; GRAPEFRUIT; OBESITY complications; ADIPOSE tissues; ANALYSIS of variance; ANIMAL experimentation; CELL physiology; DIET; ENZYMES; FATTY acids; GENE expression; HEALTH promotion; LIPIDS; MICE; WEIGHT loss; DESCRIPTIVE statistics; DIAGNOSIS; THERAPEUTICS
- Publication
American Journal of Chinese Medicine, 2018, Vol 46, Issue 3, p601
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X18500313