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- Title
Conjugated linoleic acids modulate UVR-induced IL-8 and PGE2 in human skin cells: potential of CLA isomers in nutritional photoprotection.
- Authors
Amy Storey; Julia S. Rogers; Francis McArdle; Malcolm J. Jackson; Lesley E. Rhodes
- Abstract
Conjugated linoleic acids (CLA), derivatives of linoleic acid found in food products, inhibit chemically induced skin cancers in mice. However, their potential photoprotective properties remain unexplored. We examined whether CLA may modulate ultraviolet radiation (UVR)-induced secretion of interleukin (IL)-8 and prostaglandin E2 (PGE2), mediators implicated in UVR-induced inflammation and carcinogenesis, in human skin cells. Since tumour necrosis factor (TNF)-α is an early mediator of UVR effects, we also examined influence of CLA on TNF-α-induced mediator release. HaCaT keratinocytes were supplemented with CLA isomers cis-9-trans-11 (c9,t11-CLA; ≥90%), trans-10-cis-12 (t10,c12-CLA; ≥90%) or all trans-trans isomers (tt-CLA; 23.7%) in tetrahydrofuran/fetal calf serum (THF/FCS) or THF/FCS control. Supplementation of keratinocytes with c9,t11-CLA reduced Ultraviolet B(UVB)-induced IL-8 from 37 113 ± 2903 pg/ng protein in control cells to 14 167 ± 2063 pg/ng protein (P t10,c12-CLA reduced UVB-induced IL-8 to 9786 ± 1291.5 pg/ng protein (P t10,c12-CLA and tt-CLA inhibited TNF-α-induced IL-8 from 11 669 ± 1692 pg/ng protein in control cells to 5540 ± 191 (P P 2 release was reduced by tt-CLA supplementation, from 4.8 ± 1.2 to 1.6 ± 0.8 pg/mg protein (P t10,c12-CLA to 8.8 ± 1 pg/mg protein (P 2 release was further explored in CCD922SK dermal fibroblasts. CLA isomers reduced UVB-induced PGE2 in fibroblasts, reaching significance with c9,t11-CLA (98 ± 5 falling to 0 pg/mg protein, P in vitro. CLA-containing foods have potential in photoprotection; the cutaneous effects of individual isomers warrant clinical study.
- Subjects
CANCER treatment; LINOLEIC acid; SKIN cancer; MEDICAL research
- Publication
Carcinogenesis, 2007, Vol 28, Issue 6, p1329
- ISSN
0143-3334
- Publication type
Article
- DOI
10.1093/carcin/bgm065