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- Title
Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy.
- Authors
Wong, Terence T. W.; Ruiying Zhang; Chi Zhang; Hsun-Chia Hsu; Maslov, Konstantin I.; Lidai Wang; Junhui Shi; Ruimin Chen; Shung, K. Kirk; Qifa Zhou; Wang, Lihong V.
- Abstract
Three-dimensional (3D) optical imaging of whole biological organs with microscopic resolution has remained a challenge. Most versions of such imaging techniques require special preparation of the tissue specimen. Here we demonstrate microtomy-assisted photoacoustic microscopy (mPAM) of mouse brains and other organs, which automatically acquires serial distortion-free and registration-free images with endogenous absorption contrasts. Without tissue staining or clearing, mPAM generates micrometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achieved by label-free simultaneous sensing of DNA/RNA, hemoglobins, and lipids. mPAM provides histology-like imaging of cell nuclei, blood vessels, axons, and other anatomical structures, enabling the application of histopathological interpretation at the organelle level to analyze a whole organ. Its deep tissue imaging capability leads to less sectioning, resulting in negligible sectioning artifact. mPAM offers a new way to better understand complex biological organs.
- Subjects
THREE-dimensional imaging; MICROSCOPY; CELL imaging; OPTICAL images; BLOOD vessels; CELL nuclei
- Publication
Nature Communications, 2017, Vol 8, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-017-01649-3