We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Anti-tumor and apoptosis studies of plant extract derived from Cynanchum-callialatum Ham. Ex Wight in breast cancer cell lines and in vivo animal models.
- Authors
Karthikeyan, M.
- Abstract
Cynanchum is a genus of about 300 species including some small climbers; Herbs twining to 4 m. Stems pubescent along 1 side, belongs to the family Asclepiadaceae (Milk weed family). Antiproliferative, apoptosis induction studies, anti-inflammatory studies and invivo anticancer studies has been studied by various methods and models with CCEE. The Cynanchumcallialatumethyl acetate extracts (CCEE) showed potential cyctoxicity against MCF-7 cell lines. The results showed that MCF-7 cell proliferation was significantly inhibited by CCEE with the IC50 value 300 μg/ml. The standard drug doxorubicin with the IC50 value of 2.38 μg/ml.The effect CCEE on cell cycle was studied using flow cytometry in MCF-7 cell lines. The percentage of cell in various phases like G0/G1, S and G2/M phase of the cell were calculated. The results shows that the CCEE inhibited cells in the G0/G1 phase in the MCF-7 cells. Doxorubicin arrested the G2/M phase of the cell in MCF-7 cells. Apoptotic changes in cells may be studied using AO/EB fluorescent staining. The apoptotic effect of CCEE was studied in MCF-7cell lines and the percentage of apoptotic cells were calculated.The levels of p-53, phospho-p53, Bad, phospho-Bad, cleaved caspase 3 and cleaved PARP in MCF-7cell lines were studied by ELISA method. There was a slight decrease in p53 in doxorubicin. The markers were significantly increased by all the treatments when compared to the normal control cells. Lifespan of ascites tumor bearing animals induced by EAC cells was found to be increased by CCEE in 200mg/kg and 400mg/kg treatment. In CCEE 400mg/kg group there was a significant increase in lifespan (p<0.001) of 72.38%compared to EAC control. Similar findings was reported in DLA solid tumor model. Therefore, the effect of PCEE on the inhibition of IL-1β and TNF-α was investigated. The IL-1β and TNF-α levels in supernatants of cells treated with CCEE were significantly decreased compared with the LPS group, in a dose-dependent manner. Our research indicates that these this plants possess anticancer and anti-inflammatory activities which may be due to the presence of active phytoconstituents flavonoids and phenolic compounds. It can be concluded that the plant extracts may be a source of new compounds for anticancer and anti-inflammatory.
- Subjects
PLANT extracts; CANCER cells; CELL lines; BREAST cancer; ANIMAL models in research; ASCITIC fluids
- Publication
International Journal of Pharmacy & Life Sciences, 2020, Vol 11, Issue 7, p48
- ISSN
0976-7126
- Publication type
Article