We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Analysis of matrix metalloproteinase-8 levels in gingival crevicular fluid and whole mouth fluid among smokers and nonsmokers using enzyme-linked immune-sorbent assay and a novel chair-side test.
- Authors
Akbari, Ghousia; Venkatesh Prabhuji, Munivenkatappa Lakshmaiah; Karthikeyan, Bangalore Vardhan; Raghunatha, Kanugondappa; Narayanan, Roopalakshmi
- Abstract
Aim: To indigenously prepare a chair-side test kit for investigating and comparing the matrix metalloproteinase (MMP)-8 levels in gingival crevicular fluid (GCF) and saliva in patients with healthy periodontium, gingivitis and chronic periodontitis in smokers and nonsmokers. To validate the diagnostic accuracy of indigenously prepared chair-side test against enzyme-linked immune-sorbent assay (ELISA). Furthermore, to assess the effect of nonsurgical periodontal therapy (NSPT) on the levels of MMP-8 in GCF and saliva among the test groups. Materials and Methods: GCF and saliva were collected from 250 subjects. The study population were divided into five groups; health periodontium-nonsmokers (Group 1; n = 50), chronic gingivitis-nonsmokers (Group 2; n = 50), chronic periodontitis-nonsmokers (Group 3; n = 50), chronic gingivitis-smokers (Group 4; n = 50), chronic periodontitis-smokers (Group 5; n = 50). A chair-side test kit was indigenously prepared using polyclonal antibodies (principle of immunochromatography) to detect the MMP-8 levels, and it was validated against ELISA at baseline and 3 months after NSPT. Results: The chair-side test detected MMP-8 levels with a sensitivity and specificity in accordance with ELISA. MMP-8 levels at baseline were higher in Group 2 and Group 3 as compared to controls (P < 0.05), and decreased after therapy (P < 0.05). MMP-8 levels in GCF were greater than in saliva for all the groups, indicating GCF to be a better sample to detect the MMP levels. Conclusion: The chair-side test detected MMP-8 levels accurately making it a viable chair side diagnostic tool. It was effective for early diagnosis of the periodontal disease among high-risk population such as smokers.
- Subjects
MATRIX metalloproteinases; MATRIX metalloproteinase inhibitors; THERAPEUTIC use of protease inhibitors; SORBENT testing; PERIODONTAL disease treatment; THERAPEUTICS
- Publication
Journal of Indian Society of Periodontology, 2015, Vol 19, Issue 5, p525
- ISSN
0972-124X
- Publication type
Article
- DOI
10.4103/0972-124X.162201