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- Title
Genotyping of virulent Escherichia coli obtained from poultry and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction.
- Authors
Sekhar, M. Soma; Sharif, N. Mohammad; Rao, T. Srinivasa; Metta, M.
- Abstract
Aim: The aim of this study was to characterize virulent Escherichia coli isolated from different poultry species and poultry farm workers using enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) genotyping. Materials and Methods: Fecal swabs from different poultry species (n=150) and poultry farm workers (n=15) were analyzed for E. coli and screened for virulence genes (stx1, stx2, eaeA, and hlyA) by multiplex PCR. Virulent E. coli was serotyped based on their "O" antigen and then genotyped using ERIC-PCR. Results: A total of 134 E. coli isolates (122/150 from poultry and 12/15 from farm workers) were recovered. Virulence genes were detected in a total of 12 isolates. Serological typing of the 12 virulent E. coli revealed nine different serotypes (O2, O49, O60, O63, O83, O101, O120, UT, and Rough). ERIC-PCR genotyping allowed discrimination of 12 virulent E. coli isolates into 11 ERIC-PCR genotypes. The numerical index of discrimination was 0.999. Conclusion: Our findings provide information about the wide genetic diversity and discrimination of virulent E. coli in apparently healthy poultry and poultry farm workers of Andhra Pradesh (India) based on their genotype.
- Subjects
VIRULENCE of Escherichia coli; BACTERIAL genetics; ESCHERICHIA coli; POULTRY farms; AGRICULTURAL laborers; POLYMERASE chain reaction; SEROTYPING; ISOLATION of biotechnological microorganisms; GENOTYPES
- Publication
Veterinary World, 2017, Vol 10, Issue 11, p1292
- ISSN
0972-8988
- Publication type
Article
- DOI
10.14202/vetworld.2017.1292-1296