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- Title
Regulation of the ovarian inflammatory response at ovulation by nuclear progesterone receptor.
- Authors
Akison, Lisa K.; Robertson, Sarah A.; Gonzalez, Macarena B.; Richards, JoAnne S.; Smith, C. Wayne; Russell, Darryl L.; Robker, Rebecca L.
- Abstract
Problem: The nuclear progesterone receptor (PGR) transcription factor is essential for ovulation; however, the exact mechanisms by which PGR controls ovulation are not known. The aim of this study was to determine whether PGR regulates inflammatory mediators in the ovary. Method of Study: Ovaries from mice lacking PGR (PRKO) and heterozygous PR+/− littermates were subjected to microarray analysis of a large panel of inflammatory genes. Immune cell subsets were detected by gene expression; and neutrophils by immunohistochemistry and chemotaxis assay. Results: PRKO ovaries exhibited dysregulated expression of vasodilator (<italic>Edn1</italic>), cytokine (<italic>Il‐6</italic>,<italic> Tgfb1</italic>), adhesion receptor (<italic>Cd34</italic>), apoptotic factor (<italic>Bax</italic>) and transcription factors (<italic>Nfkb2</italic>,<italic> Socs1</italic>,<italic> Stat3</italic>). <italic>Ptgs2</italic> was also reduced in PRKO ovaries, but mRNA and protein were not different in granulosa cells. There were reduced neutrophils in ovaries of PRKO mice at ovulation; however, chemotaxis assays showed PRKO neutrophils migrate normally and that PRKO ovarian extracts exhibit chemotactic properties in vitro. Conclusion: Specific inflammatory mediators are altered in the ovaries of PRKO mice indicating that progesterone regulates features of inflammation at ovulation.
- Subjects
T cells; PROGESTERONE receptors; IMMUNOHISTOCHEMISTRY; GENE expression; NEUTROPHILS; CHEMOTAXIS
- Publication
American Journal of Reproductive Immunology, 2018, Vol 79, Issue 6, p1
- ISSN
1046-7408
- Publication type
Article
- DOI
10.1111/aji.12835