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- Title
Intermittent white noise exposure is associated with rat cochleae damage and changes in the gene expression.
- Authors
Hotkani, Maryam Mirzaei; Monazzam Esmaeilpoor, Mohammad Reza; Khadem, Monireh; Garmaroudi, Amir Abbasi; Inanloorahatloo, Kolsoum
- Abstract
Background: Noise, a physical factor in most work environments, has many efects on human health. Exposure to excessive noise can modify the expression of associated genes with NIHL. The aim of this study to elucidate changes in expression of GJB2 and SLC26A4 after exposure to intense noise which are the most frequent causing genes to apparent autosomal recessive non-syndromic hearing loss. Methods: In this experimental and case–control study, 17 male Wistar rats were randomly divided into exposure groups (n=12) and without exposure (n=5). First group was exposed to noise (90–120 dB, 70 Hz–16 kHz, 8 h/day) for 3–6 days. Cochlear biopsies performed 1 h and 1 week post-exposure, relative gene expression levels were calculated using 2− Δ ΔCt. From each group, one ear was stained by hematoxylin and eosin method for histopathological survey. Real-time PCR technique took place, and gene expression data were normalized by GAPDH gene. One-way ANOVA test was performed with a signifcance level of 0.05 by GraphPad prism software. Results: Both GJB2 and SLC26A4 in all groups were down-regulated after exposure compared to their controls. Fold changes in the highest times were related to 1 week after 6 days of exposure, 0.052 and 0.015, respectively. Serious damages occurred in diferent parts of cochlea, and they were more severe after 6 days and 1-week later. Conclusion: It is expected that if the hearing threshold tests be performed before/after exposure and considering longer post-exposure times, subsequently, the expression of these genes does not return to basal level, and irrecoverable damage to the cochlea, progressive and irreversible ARNSHL will be expected.
- Subjects
RECESSIVE genes; GENE expression; WHITE noise; COCHLEA; LABORATORY rats; ONE-way analysis of variance
- Publication
Egyptian Journal of Medical Human Genetics, 2022, Vol 23, p1
- ISSN
1110-8630
- Publication type
Article
- DOI
10.1186/s43042-022-00317-6