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- Title
CRISPR base editing applications for identifying cancer-driving mutations.
- Authors
Pal, Martin; Herold, Marco J.
- Abstract
CRISPR base editing technology is a promising genome editing tool as (i) it does not require a DNA template to introduce mutations and (ii) it avoids creating DNA double-strand breaks, which can lead to unintended chromosomal alterations or elicit an unwanted DNA damage response. Given many cancers originate from point mutations in cancer-driving genes, the application of base editing for either modelling tumour development, therapeutic editing, or functional screening is of great promise. In this review, we summarise current DNA base editing technologies and will discuss recent advancements and existing hurdles for its usage in cancer research.
- Subjects
DOUBLE-strand DNA breaks; CRISPRS; GENOME editing; DNA damage
- Publication
Biochemical Society Transactions, 2021, Vol 49, Issue 1, p269
- ISSN
0300-5127
- Publication type
Article
- DOI
10.1042/BST20200550