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- Title
SALIVARY FREE CATECHOLAMINES METABOLITES AS POSSIBLE BIOCHEMICAL MARKERS IN PHEOCHROMOCYTOMA DIAGNOSIS.
- Authors
Stefanescu, A. M.; Schipor, S.; Paun, D. L.; Dumitrache, C.; Badiu, C.
- Abstract
Background: Salivary monitoring of hormone levels has many advantages over the more conventional serum/plasma analysis. Salivary free metanephrines (MN) and normetanephrines (NMN) could precise biochemical diagnosis of pheochromocytoma (PHEO) as an alternative to plasma metabolites. Subjects and methods: The prospective case-control study included a group of 30 patients confirmed with PHEO an age-matched control group of 70 normotensive subjects. The PHEO diagnosis was suspected on clinical ground and confirmed by imaging studies and classical neuroendocrine markers. Free plasma and salivary NMN and MN were assayed using enzyme immunoassay for both metabolites. Results: In tumor cases all metabolites were increased. As expected, values for all 4 parameters (mean±SEM) differed significantly in tumor group vs. normal group: free plasma normetanephrines (NMNp): 1514.16 ± 282.97 pg/mL vs 47.82±2.52 pg/mL; free salivary normetanephrines (NMNs): 663.63±168.47 pg/mL vs 44.98± 2.47 pg/mL; free plasma metanephrines (MNp): 445.20 ± 99.92 pg/mL vs 18.87±1.03 pg/mL; free salivary metanephrines (MNs):206.60±91.48 pg/mL vs 14.47±0.72 pg/mL with significant correlations in all 100 subjects. Passing & Bablok regression showed no significant deviation from linearity in Elisa assay of NMNs vs NMNp; a significant deviation from linearity existed in Elisa assay of MNs vs MNp. Cut-off values, sensitivity and specificity for all 4 parameters were calculated by ROC analysis. Plasma and salivary normetanephrines proved similar sensitivity (100%) and specificity (100%). Pairwise comparison of ROC curves areas showed no significant differences between NMNp vs NMNs and MNp vs MNs. Ten cases were investigated post-surgery. All 4 parameters showed no significant differences vs. control group. Conclusions: Salivary free normetanephrines could be used as a nonstressful marker for diagnosis purpose in pheochromocytoma proving similar sensitivity and specificity as plasma free normetanephrines.
- Subjects
CATECHOLAMINES; BIOMARKERS; PHEOCHROMOCYTOMA; ENZYME-linked immunosorbent assay; SALIVA analysis; METABOLITES; DIAGNOSIS
- Publication
Acta Endocrinologica (1841-0987), 2011, Vol 7, Issue 4, p431
- ISSN
1841-0987
- Publication type
Article
- DOI
10.4183/aeb.2011.431