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- Title
Cytokinesis mediated through the recruitment of cortexillins into the cleavage furrow.
- Authors
Weber, Igor; Gerisch, Günther; Heizer, Christina; Murphy, John; Badelt, Kim; Stock, Alexander; Schwartz, Jean-Marc; Faix, Jan
- Abstract
The fact that substrate-anchored Dictyostelium cells undergo cytokinesis in the absence of myosin II underscores the importance of other proteins in enabling the cleavage furrow to constrict. Cortexillins, a pair of actin-bundling proteins, are required for normal cleavage. They are targeted to the incipient furrow in wild-type and, more prominently, in myosin II-null cells. No other F-actin bundling or cross-linking protein tested is co-localized. Green fluorescent protein fusions show that the N-terminal actin-binding domain of cortexillin I is dispensable and the C-terminal region is sufficient for translocation to the furrow and the rescue of cytokinesis. Cortexillins are suggested to have a targeting signal for coupling to a myosin II-independent system that directs transport of membrane proteins to the cleavage furrow.
- Subjects
DICTYOSTELIUM; CYTOKINESIS; MYOSIN; PROTEINS; SCISSION (Chemistry); ACTIN; MEMBRANE proteins
- Publication
EMBO Journal, 1999, Vol 18, Issue 3, p586
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/18.3.586