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- Title
Absence of Oxidative and Inflammatory Response Following Orange Juice: A Comparison with Glucose Intake.
- Authors
Dandona, Paresh; Ghanim, Husam; Viswanathan, Prabhakar; Upadhyay, Manish; Sia, Ching Ling; Mohanty, Priya
- Abstract
We have previously shown that the intake of a 300 kcal from glucose or a 900 kcal from a fast food meal induces a significant increase in reactive oxygen species (ROS) generation and nuclear factor κ-B (NFκB) binding in the circulating mononuclear cells (MNC) in healthy normal weight subjects. In this study we investigated wither the replacement of glucose with an equicaloric drink of orange juice (OJ) (300 kcal) alone or with a 900 kcal fast food breakfast will protect from the increase in oxidative stress and inflammation. Two groups (24 subjects each) of normal weight healthy subjects were asked to ingest the drinks alone or with the meal and were monitored prior to and for 5 hr following the meal. Although insulin concentrations increased significantly by 7-11 folds (P<0.001) following both glucose and OJ intake with or without the meal, there was a significant increase in glucose concentrations (P<0.01) only when glucose was ingested alone or with the meal while it was absent when OJ replaced glucose. There was a significant increase in ROS generation by MNC and PMN, (P< 0.05) within 3 hours of glucose intake alone or following glucose or OJ with the meal while OJ alone did not cause any increase in ROS generation. However, the increase in ROS generation by PMN induced by glucose and the meal was significantly greater when compared to that induced by OJ and the meal (P<0.05). Glucose, but not OJ, caused a significant increase in NFκB binding (P<0.05). On the other hand, there was no significant change in NFκB binding when the meal was added to either drink. Also, glucose intake alone or with the meal caused a significant increase in protein levels of p47[sup phox] and p38 MAP kinase (P<0.05) and in the mRNA expression of TNFα, MMP9 and SOCS-3 (P<0.02) in the MNC while OJ intake alone or with the meal did not induce any significant changes in the levels of these inflammatory mediators except for a rise (P<0.05) in TNFα mRNA expression following OJ alone. We conclude that OJ intake does not induce and oxidative or inflammatory response when compared to glucose intake. Also, that when added to a fast food meal, OJ protects the MNC from the rise in inflammatory mediators and to a lesser extend oxidative stress when compared to glucose intake.
- Subjects
GLUCOSE; ORANGE juice; NF-kappa B; REACTIVE oxygen species; OXIDATIVE stress; MESSENGER RNA; PROTEINS
- Publication
Diabetes, 2007, Vol 56, pA449
- ISSN
0012-1797
- Publication type
Article