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- Title
Interaction between Tumor-Associated Dendritic Cells and Colon Cancer Cells Contributes to Tumor Progression via CXCL1.
- Authors
Hsu, Ya-Ling; Chen, Yi-Jen; Chang, Wei-An; Jian, Shu-Fang; Fan, Hsiao-Li; Wang, Jaw-Yuan; Kuo, Po-Lin
- Abstract
Crosstalk of a tumor with its microenvironment is a critical factor contributing to cancer development. This study investigates the soluble factors released by tumor-associated dendritic cells (TADCs) responsible for increasing cancer stem cell (CSC) properties, cell mobility, and epithelial-to-mesenchymal transition (EMT). Dendritic cells (DCs) of colon cancer patients were collected for phenotype and CXCL1 expression by flow cytometry and Luminex assays. The transcriptome of CXCL1-treated cancer cells was established by next generation sequencing. Inflammatory chemokine CXCL1, present in large amounts in DCs isolated from colon cancer patients, and SW620-conditioned TADCs, enhance CSC characteristics in cancer, supported by enhanced anchorage-independent growth, CD133 expression and aldehyde dehydrogenase activity. Additionally, CXCL1 increases the metastatic ability of a cancer by enhancing cell migration, matrix metalloproteinase-7 expression and EMT. The enhanced CXCL1 expression in DCs is also noted in mice transplanted with colon cancer cells. Transcriptome analysis of CXCL1-treated SW620 cells indicates that CXCL1 increases potential oncogene expression in colon cancer, including <italic>PTHLH</italic>, <italic>TYRP1</italic>, <italic>FOXO1</italic>, <italic>TCF4</italic> and <italic>ZNF880</italic>. Concurrently, CXCL1 displays a specific microRNA (miR) upregulated by the prototypical colon cancer onco-miR miR-105. Analysis of publicly available data reveals CXCL1-driven oncogenes and miR-105 have a negative prognostic impact on the outcome of colon cancer. This study indicates a new mechanism by which the colon cancer milieu exploits DC plasticity to support cancer progression.
- Subjects
DENDRITIC cells; COLON cancer; CANCER invasiveness; CANCER stem cells; CHEMOKINES
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 8, p2427
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19082427