We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Molecular and genomic features of Mycobacterium bovis strain 1595 isolated from Korean cattle.
- Authors
Narae Kim; Yunho Jang; Jin Kyoung Kim; Soyoon Ryoo; Ka Hee Kwon; Miso Kim; Shin Seok Kang; Hyeon Seop Byeon; Hee Soo Lee; Young-Hee Lim; Jae-Myung Kim
- Abstract
The aim of this study was to investigate the molecular characteristics and to conduct a comparative genomic analysis of Mycobacterium (M.) bovis strain 1595 isolated from a native Korean cow. Molecular typing showed that M. bovis 1595 has spoligotype SB0140 with mycobacterial interspersed repetitive units-variable number of tandem repeats typing of 4-2-5-3-2-7-5-5-4-3-4-3-4-3, representing the most common type of M. bovis in Korea. The complete genome sequence of strain 1595 was determined by single-molecule real-time technology, which showed a genome of 4351712 bp in size with a 65.64% G + C content and 4358 protein-coding genes. Comparative genomic analysis with the genomes of Mycobacterium tuberculosis complex strains revealed that all genomes are similar in size and G + C content. Phylogenetic analysis revealed all strains were within a 0.1% average nucleotide identity value, and MUMmer analysis illustrated that all genomes showed positive collinearity with strain 1595. A sequence comparison based on BLASTP analysis showed that M. bovis AF2122/97 was the strain with the greatest number of completely matched proteins to M. bovis 1595. This genome sequence analysis will serve as a valuable reference for improving understanding of the virulence and epidemiologic traits among M. bovis isolates in Korea.
- Subjects
KOREA; MYCOBACTERIUM bovis; CATTLE genetics; GENOMICS; TANDEM repeats
- Publication
Journal of Veterinary Science, 2017, Vol 18, p333
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2017.18.S1.333