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- Title
Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model.
- Authors
Dong-Hun Lee; Jong-In Kim; Jae-Won Lee; Wook-Hun Chung; Jae-Keun Park; Yu-Na Lee; Jin Soo Han; Hwi-Yool Kim; Sang-Won Lee; Chang-Seon Song
- Abstract
The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.
- Subjects
RESPIRATORY infections; BRONCHOALVEOLAR lavage; INFLUENZA viruses; VIRAL replication; VIRAL tropism; FERRETS as laboratory animals; DIAGNOSIS
- Publication
Journal of Veterinary Science, 2014, Vol 15, Issue 3, p439
- ISSN
1229-845X
- Publication type
Article
- DOI
10.4142/jvs.2014.15.3.439