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- Title
Distribution of MICB diversity in the Zhejiang Han population: PCR sequence-based typing for exons 2-6 and identification of five novel MICB alleles.
- Authors
Ying, Yanling; He, Yanmin; Tao, Sudan; Han, Zhedong; Wang, Wei; Chen, Nanying; He, Junjun; Zhang, Wei; He, Ji; Zhu, Faming; Lv, Hangjun
- Abstract
The polymorphism of major histocompatibility complex class I chain-related gene B ( MICB) and variations in MICB alleles in a variety of populations have been characterized using several genotyping approaches. In the present study, a novel polymerase chain reaction sequence-based typing (PCR-SBT) method was established for the genotyping of MICB exons 2-6, and the allelic frequency of MICB in the Zhejiang Han population was investigated. Among 400 unrelated healthy Han individuals from Zhejiang Province, China, a total of 20 MICB alleles were identified, of which MICB* 005: 02: 01, MICB* 002: 01: 01, and MICB* 004: 01: 01 were the most predominant alleles, with frequencies of 0.57375, 0.1225, and 0.08375, respectively. Nine MICB alleles were detected on only one occasion, giving a frequency of 0.00125. Of the 118 distinct MICB ∼ HLA- B haplotypes identified, 42 showed significant linkage disequilibrium ( P < 0.05). Haplotypes MICB* 005: 02: 01 ∼ B* 46: 01, MICB* 005: 02: 01 ∼ B* 40: 01, and MICB* 008 ∼ B* 58: 01 were the most common haplotypes, with frequencies of 0.0978, 0.0761, and 0.0616, respectively. Five novel alleles, MICB* 005: 07, MICB* 005: 08, MICB* 027, MICB* 028, and MICB* 029 were identified. Compared with the MICB* 005: 02: 01 sequence, a G > A substitution was observed at nucleotide position 210 in MICB* 005: 07, and a 1,134 T > C substitution in MICB* 005: 08 and an 862 G > A substitution in MICB* 027 were detected. In addition, it appears that MICB* 028 probably arose from MICB* 004: 01: 01 with an A to G substitution at position 1,147 in exon 6. MICB* 029 had a G > T transversion at nucleotide position 730 in exon 4, compared with that of MICB* 002: 01: 01. On the basis of the new PCR-SBT assay, these observed results demonstrated MICB allelic variations in the Zhejiang Han population.
- Subjects
ZHEJIANG Sheng (China); MAJOR histocompatibility complex; EXONS (Genetics); ALLELES; POLYMERASE chain reaction; HUMAN genetic variation; GENETIC polymorphisms
- Publication
Immunogenetics, 2013, Vol 65, Issue 7, p485
- ISSN
0093-7711
- Publication type
Article
- DOI
10.1007/s00251-013-0699-4